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KMID : 1094720100150050754
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Biotechnology and Bioprocess Engineering
2010 Volume.15 No. 5 p.754 ~ p.760
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Exploration of glycosylated flavonoids from metabolically engineered E. coli
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Simkhada Dinesh
Kurumbang Nagendra Prasad
Lee Hei-Chan
Sohng Jae-Kyung
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Abstract
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Flavonoids glycosylated with UDP-glucuronic acid and UDP-xylose are spatially distributed in nature. To produce these glycosides, E. coli was engineered to overexpress biosynthetic gene clusters of UDP-sugars (galU from E. coli K12, UDP-glucose dehydrogenase (calS8), and UDP-glucuronic acid decarboxylase (calS9) from Micromonospora echinospora spp. calichensis). Flavonoids were glycosylated by overexpression of the glycosyltransferase gene (atGt-5) from Arabidopsis thaliana. Finally, metabolically engineered host E. coli (US89Gt-5) was generated. Production of flavonoid glycosides was observed in a biotransformation system consisting of flavonoids (naringenin and quercetin) exogenously fed to host cells. The glycosylated derivatives 7-O-glucuronyl naringenin (m/z+ 449), 7-O-xylosyl naringenin (m/z+ 405), and 7-O-glucuronyl quercetin (m/z+ 479) were detected and confirmed by ESI-MS/MS, ESI-MS/MS and LC/MS-MS analysis, respectively.
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KEYWORD
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E. coli (US89Gt-5), metabolic engineering, UDP-sugars, 7-O-glucuronyl naringenin, 7-O-xylosyl naringenin, 7-O-glucuronyl quercetin etc.
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